✍️ Edited by Farhan Ahmed, Editor, MedTestBD · Last updated: August 2026
What is the ANA Test?
The Anti-Nuclear Antibody (ANA) test detects antibodies that the immune system produces against the body's own cell nuclei. These autoantibodies are a hallmark of autoimmune diseases — conditions where the immune system mistakenly attacks healthy tissue. ANA is used as a screening test for a range of autoimmune conditions, most notably Systemic Lupus Erythematosus (SLE). A positive ANA alone does not diagnose disease — further specific tests are needed.
Why is the ANA Test Done?
- To screen for Systemic Lupus Erythematosus (SLE or lupus) — ANA is positive in over 95% of lupus patients
- To investigate symptoms such as joint pain, skin rashes, unexplained fever, fatigue, or hair loss that suggest an autoimmune cause
- To evaluate Sjögren's syndrome, mixed connective tissue disease, scleroderma, and other autoimmune conditions
- To investigate Raynaud's phenomenon — colour changes in fingers triggered by cold
- As a first-line autoimmune screen alongside CRP, ESR, and CBC
Normal ANA Results
| Result | Titre | Interpretation |
|---|---|---|
| Negative | Below 1:40 | Autoimmune disease unlikely |
| Low positive | 1:40 – 1:80 | May be seen in healthy individuals — clinical correlation needed |
| Positive | 1:160 or above | Significant — further specific antibody testing recommended |
| Strongly positive | 1:320 or above | Strongly associated with autoimmune disease |
Up to 20% of healthy individuals can have a low-positive ANA at 1:40. A positive ANA at higher titres, combined with specific symptoms, should prompt further testing for anti-dsDNA, anti-Sm, anti-Ro, and anti-La antibodies to identify the specific autoimmune condition.
How to Prepare for the ANA Test
- No fasting is required
- Inform your doctor about steroids or immunosuppressive medications you are taking — they can suppress antibody levels and cause a false negative
- Inform the doctor about any ongoing infections, as acute infections can temporarily cause a positive ANA
- A simple blood sample is drawn from a vein in your arm
- Results are usually available within 24–48 hours
ANA Test Price in Bangladesh
ANA test prices vary between diagnostic centers across Bangladesh. Compare ANA test prices on MedTestBD →
Normal ranges cited on this page are based on MedlinePlus and WHO guidance. Prices are collected from public sources and may change — confirm with the facility before visiting. This information is for general reference only and is not medical advice. Always consult a qualified physician.
